I had similar problems with uploading my game play to Dtube. What helped me was to decrease the bit rate from 50Mbps to 15Mbps while recording with OBS. That's a reasonable bit rate which keeps the quality at an acceptable level. I've also heard it's safer to keep the video size under 1 gig if possible.
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Yeah, I've heard about the 1 GB limit, but I've uploaded videos of more than 1 GB previously, so I can't tell how accurate that information is actually.
When it comes to the bitrate:
As I stream on Twitch whenever I play, I use 5000 bitrate. I never record with OBS, but thanks for letting me know. Good to know for future videos! :)