Mr Trump's unexpected Twitter declaration on Wednesday has been met with feedback from rights gatherings.
Representative Sarah Sanders said the organization would work close by the Pentagon to choose how to continue.
It is not yet clear how the declaration will influence current transgender administration work force.
"The United States government won't acknowledge or enable transgender people to serve in any way in the US military," Donald Trump tweeted.
"Our military must be centered around definitive and overpowering triumph and can't be troubled with the colossal restorative expenses and interruption that transgender in the military would involve."
Asked at a press preparation if troops on front lines would be promptly sent back, White House representative Sarah Sanders said the approach still couldn't seem to be worked out.
"The choice depends on a military choice. It's not intended to be much else besides that," she said.
The mystery life of a transgender aviator
Notwithstanding, some US media outlets scrutinized the spending avocation. The Washington Post attracted consideration regarding an examination that the US military spends nearly $42m a year on the erectile brokenness pharmaceutical Viagra - a few times the aggregate assessed cost of transgender restorative help.
In the mean time, Poltico reports that the move was incited by dangers from Republican hardliners over a spending charge which would give financing to Mr Trump's guaranteed military spending and fringe divider designs.
One Republican official had just tabled an alteration to the spending bill to keep the military paying for transgender surgical methodology.
Armed force reservist Rudy Akbarian, in Los Angeles, stated: "My heart dropped a tad bit, you know. It hurt."
"Not every person reacted well in the wake of learning I was transitioning," he said. "Be that as it may, subsequent to investing energy in missions and acknowledging we as a whole offer a similar love for the nation, we cooperated and took care of business.
"The separation I'm confronting now is from those outside the military - not the general population who work with me."
What is the response from resistance experts?
Mr Trump said his choice depended on counsel with his commanders, however there has been a blended response.
Previous Defense Secretary Ash Carter, who lifted the boycott a year ago under President Obama, stated: "To pick benefit individuals on different grounds than military capabilities is social approach and has no place in our military."
Administrator of the Senate Armed Services Committee, Republican John McCain, said real strategy declarations ought not be made by means of Twitter.
"Any American who meets current therapeutic and availability models ought to be permitted to keep serving," he included.