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RE: Methods, Methods, Methods (My Research, Vol. 5)

in #science6 years ago

Our lab wants to cut down on EtBr usage so they are doing it like this. We have a basin with 1l water and a few drops of EtBr (so that the water is pink). The gel is then put into that for 15 minutes (10 for RNA). Then for a moment put in a basin with pure water (to get rid of leftover EtBr, we don't want it everywhere). Then you can put it under the UV light. Takes a bit longer but cuts down the toxic stuff you're exposed to!