Spectral Phasor for ImageJ
Software used for the analysis of hyperstacks
Screenshots
Hunter's comment
The idea behind the software is to reduce the dimensionality of the image hyperstacks (50+ images). Some examples could include multichannel microscopy, FLIM, FTIR and similar techniques.
Dimensionality reduction is achieved by transforming the images to another domen, represented by phasor.
Actually, it's very intuitive, further from the centre - narrower component, Closer to the centre - broader the components. Moving around the circle equals moving the component on X-axis.
Pros: works fine with ImageJ
Cons: for trivial examples, like the one presented. They used 3 fluorescence dyes with very different properties. I used the software for a much more difficult problem and NMF performed better.
Anyway, it's cool and unusual way of thinking, outside the box that you could exploit in your research routine.
My review score: 8.5 of 10
Link
http://www.spechron.com/Spectral%20Phasor-Download.aspx
Contributors
Hunter: @alexs1320
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When people ask me what do you do for a living:
ImageJ is my go to recommendation for scientific image analysis software. Free and relatively easy to use. Good to see more software enhancing its capability.
And a lot of free plug-ins :)
I used ImageJ ages ago in my undergrad research project for amylase analysis. Cool to see it still being used.
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