Insect Cell Lines
Biotechnology Vaccines
Spike Protein Folding
Self Assemble Nanoparticle Structures
Baculovirus
Recombinant Proteins
Viruses-like Particles (VLPs)
Polyhedrin Promoter
Lepidoptera
lepidopteran Cells
Baculoviral Polh Locus
Heterologous Protein
Autographa Californica Multiple Nuclear Polyhedrosis Virus (AcMNPV)
Baculovirus Expression Vector System (BEVS)
cant find anything regarding Cicadas directly, but apparently they use Moths to make spike protein.
found something very strange regarding Cicada.
Genome Instability: Hodgkinia genomes often fragment into small, highly divergent circles, suggesting they are at the edge of extinction.
Co-dependence: Hodgkinia relies on the host cicada and a second, more stable symbiont, Sulcia.
Replacement by Fungi: In some cicada species, Hodgkinia has been completely lost and replaced by fungal symbionts, a process linked to its severe genome degradation.
Yeast-Like Fungal Symbionts (YLS) in cicadas are primarily associated with Hodgkinia-free species, where they reside in the fat bodies and are believed to have evolved from entomopathogenic fungi of the genus Ophiocordyceps.
Transmission: YLS are vertically transmitted via the ovaries, often forming a "symbiont ball" in the oocytes alongside Sulcia.
Cicada Symbiont Hodgkinia is unstable and going extinct, replaced by the oldest known symbiont Ophiocordyceps.
Ophiocordyceps is the scary fungus infection, makes zombie bugs.
Ovaries..
..
the use of moths for spike & cicada having the oldest symbionts.
one of the symbionts of Cicada is failing into a Yeast-Like Fungal Symbiont.
its required for essential amino acids, but are possibly similar to Metabolic Syndrome similar to Homocysteine.
Homocysteine is dysregulated is blood clots.
my question is.. what makes a cell line Immortal? and why are insects with symbionts the most stable spike protein folding post-transcription "factories".
does immortalized have to do with Yeast-Like Symbiont research, but ultimately renamed a virus.. Baculovirus Expression Vector System?
this goes back to Plasmid Plastid Phage.. the plant-alga symbiont in Malaria, proto-HIV research.
Bats & Moths are both tied together via the most complex symbiont & viruses.
Moths & Cicada having a loose connection, but also very similar symbionts.
Moths & Bats combination are generally regarded as the most symbiont for biotechnology studies.
Moths evolved specifically with defense mechanisms to evade Bats, millions of years of symbiont interactions.
Bats have a very unusual fly blood parasite with no eyes, loaded with symbionts.
why so many symbionts & viruses in this niche little pocket?
the Cicada Variant danger level is not my concern, its a clue into the manufacturing process of biological warfare & chronic disease.
if identified by mutations patterns, then the magic trick is revealed.
i think its the symbiont & virus evolution between Bats & Moths, and Cicada symbionts do overlap with moths, and so also bats.
the symbionts weaponized to live inside human genome, mislabeled virus, gene mutations, chronic disease.
start a chain reaction of Metabolic Syndromes, cascading Homocysteine & Misfolded Proteins.